C. elegans screen for ASD risk gene–associated phenotypes

For many years, the nematode Caenorhabditis elegans has provided a powerful platform to rapidly assign functions to human genes for which they harbor orthologs. A previous study of C. elegans, funded by the SFARI Functional Screen of Autism-Associated Variants program, identified a number of associated phenotypes for a subset of genes linked to autism spectrum disorder (ASD). A new screen now reveals that changes in habituation learning are strikingly common in nematodes carrying mutations in C. elegans orthologs of ASD risk genes.

The new study was supported in part by another such targeted award and a Research Award to SFARI Investigators Catharine RankinKurt Haas, and Paul Pavlidis, who work in a mini-consortium to study the functional effects of missense and other ASD-associated mutations in a range of model organisms. Rankin, Haas, Pavlidis and their collaborators used their machine vision system to rapidly quantify the effects of mutations in nearly 100 orthologs of ASD risk genes on 26 phenotypes in C. elegans. They found that a substantial number of these mutations had specific effects on the habituation of response probability to a tactile stimulus, which in most instances resulted in impaired habituation. In follow-up experiments, they used the same paradigm to test the functionality of specific missense mutations observed in people with ASD and were also able to test for epistatic interactions between genes that had effects on the same phenotype.

In addition to identifying habituation as an ASD-associated phenotype deserving more attention in future studies, the authors also applied the auxin-inducible degradation system to test for the ability of adult re-expression of risk genes to rescue phenotypes observed upon developmental loss of their gene products. The partially successful rescue of neuroligin-associated phenotypes suggests that this C. elegans–based model may be useful as an initial screen for genes whose critical roles are not solely developmental.

C. elegans orthologs of ASD risk genes affect habituation. Graphs illustrate the sample mean distance of each genotype group from wild-type in an assay of habituation of response probability to a tactile stimulus. Strains outside the 95 percent confidence interval of the wild-type distribution are labeled and colored blue. Many more strains show impaired, rather than enhanced, habituation response. Image from McDiarmid T. A. et al. (2020).

Reference(s)


Systematic phenomics analysis of ASD-associated genes reveals shared functions and parallel networks underlying reversible impairments in habituation learning.

McDiarmid T.A., Belmadani M., Liang J., Meili F., Mathews E.A., Mullen G.P., Hendi A., Wong W.R., Rand J., Mizumoto K., Haas K., Pavlidis P., Rankin C.

Proc. Natl. Acad. Sci. U S A 117, 656-676 (January 7, 2020) PubMed

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