During doctoral training with Lin Chen at the University of Southern California, Reza Kalhor analyzed complex structural systems using high-throughput sequencing. Following on from that, as a postdoctoral research fellow in the laboratory of George Church at Harvard Medical School, Kalhor developed in vivo barcoding strategies that he used to record lineages in the mouse brain. He also helped develop in situ sequencing technologies and studied whole-brain mapping technologies.
Kalhor now plans to put these skill sets to use in addressing the challenges associated with high-resolution developmental mapping of neurons. By building upon his prior mouse barcoding and in situ sequencing technologies, Kalhor plans to create a mouse model for in vivo barcoding of the brain and integrating this model with cutting-edge single-cell sequencing technologies to obtain whole-brain lineage maps at a single-neuron resolution in both neurotypical and autism mouse models.